fluorescence spark tm 10m multimode plate reader (Tecan Systems)
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fluorescence spark tm 10m multimode plate reader
Fluorescence Spark Tm 10m Multimode Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 16796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spark+multimode+plate+reader/Spark/pmc12945581-189-29-36
Average 99 stars, based on 16796 article reviews
Fluorescence Spark Tm 10m Multimode Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 16796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spark+multimode+plate+reader/Spark/pmc12945581-189-29-36
Average 99 stars, based on 16796 article reviews
fluorescence spark tm 10m multimode plate reader - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly Article Snippet: Compounds and 5’FAM-labeled RNA probes (Genscript) were then added to yield final reaction conditions of 50 μM compound, 10 nM 5’FAM RNA, 20 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 1 mM AMP-PNP, and 1 mM MgCl2 in a total volume of 20 μL. .. Reactions were incubated at room temperature for 30 min, after which fluorescence polarization was measured on a Fluorescence:Article Title: A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly Article Snippet: Compounds and 5’FAM-labeled RNA probes (Genscript) were then added to yield final reaction conditions of 50 μM compound, 10 nM 5’FAM RNA, 20 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 1 mM AMP-PNP, and 1 mM MgCl2 in a total volume of 20 μL. .. Reactions were incubated at room temperature for 30 min, after which fluorescence polarization was measured on a Article Title: Dissolving microneedles enable effective transdermal delivery of vitamin B12 and reduce endothelial inflammation. Article Snippet: Please cite this article as: Mandeep Marwah , Emmanuella Jumbo , Pavanjeeth Balakrishnan , Lissette Sanchez-Aranguren , Hala Shokr , Sukhjinder Marwah , Hisam Siddiqi , Yukta Sameer Hindalekar , Rosie Kainth , Parmida Babaei , Sarah Junaid , Dissolving microneedles enable effective transdermal delivery of vitamin B12 and reduce endothelial inflammation, Journal of Pharmaceutical Sciences (2026), doi: https://doi.org/10.1016/j.xphs.2026.104238 Article Title: Structure-guided generative design of peptides targeting the FtsQBL divisome complex inhibit Escherichia coli cell division Article Snippet: .. Fluorescence polarization was recorded on a Tecan Binding Assay:Article Title: A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly Article Snippet: Compounds and 5’FAM-labeled RNA probes (Genscript) were then added to yield final reaction conditions of 50 μM compound, 10 nM 5’FAM RNA, 20 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 1 mM AMP-PNP, and 1 mM MgCl2 in a total volume of 20 μL. .. Reactions were incubated at room temperature for 30 min, after which fluorescence polarization was measured on a |
![HeLa cells stably expressing LgBiT were infected with endogenously HiBiT-tagged Salmonella enterica serovar Typhimurium SL1344 effector strains in three genetic backgrounds: wild-type (WT) SL1344 carrying constitutive eGFP + ( hisG 46 P tet ::gfp; PVD0002; green), the T3SS-1–deficient ΔinvA::cat mutant (PVD0316; blue), and the T3SS-2–deficient ΔssaV::cat mutant (PVD0317; red). Luminescence profiles (RLU), reflecting NanoLuc complementation following effector translocation into the host cytosol, were recorded from 2 to 26 hours post-infection (hpi). Panels show a representative subset of effectors grouped by secretion system dependence—SPI-1 (SopB, SptP, SopD; left), SPI-1/2 (GogB [25% SPI-2 contribution], SseM [56%], SseJ [95%]; middle), and SPI-2 (SteD, SseF, SspH2; right)—as determined by phase-resolved AUC analysis (see Methods and Supplementary Table S5 ). The complete set of translocation profiles for all 39 effectors is shown in . Infections were performed at a multiplicity of infection (MOI) of 50, and real-time luminescence monitoring was conducted using a Spark <t>Cyto</t> <t>400</t> multimodal plate reader under environmental control in the presence of Nano-Glo Vivazine. Luminescence values were background-subtracted (non-infected HeLa). Data points represent mean ± SD of technical quadruplicates. Individual strain identifiers and effector annotations are provided in Supplementary Table S2.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_67/10__64898_slash_2026__03__06__710167/10__64898_slash_2026__03__06__710167___F6.large.jpg)
